construction of eukaryotic expression vectors encoding cfp-10 and esat-6 genes and their potential in lymphocyte proliferation
نویسندگان
چکیده
background: mycobacterium (m.) bovis is the agent of bovine tuberculosis (tb) in a range of animal species, including humans. recent advances in immunology and the molecular biology of mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new tb vaccine. the esat-6 and cfp-10 proteins of m. bovis are important structural and functional proteins known to be important immunogens. methods: in the current study, the dnas encoding these genes were utilized in the construction of pcdna 3.1+/esat-6 and pcdna3.1+/cfp-10 plasmids. after intramuscular injection of balb/c mice with these plasmids, esat-6 and cfp-10 mrna expression was assessed by rt-pcr. mice were inoculated and boosted with the plasmids to evaluate their effects on lymphocyte proliferation. results: our results indicate the plasmids are expressed at the rna level and can induce lymphocyte proliferation. conclusion: further study is needed to characterize the effect of these antigens on the immune system and determine whether they are effective vaccine candidates against m. bovis.
منابع مشابه
Construction of Eukaryotic Expression Vectors Encoding CFP-10 and ESAT-6 Genes and Their Potential in Lymphocyte Proliferation
Background: Mycobacterium (M.) bovis is the agent of bovine tuberculosis (TB) in a range of animal species, including humans. Recent advances in immunology and the molecular biology of Mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new TB vaccine. The ESAT-6 and CFP-10 proteins of M. bovis are important structural and function...
متن کاملQuantiation of IL-4, IL-10 and IFN- Genes Expression after Immunization of Mice with CFP-10 and ESAT-6 Containing Vectors
Background: Tuberculosis is a disease with high morbidity, caused mainly by Mycobaterium tuberculosis (M.tb.). DNA vaccines show a promising future due to their unique advantages over conventional methods. The early-secreted antigen target (ESAT)-6 and culture filtrate protein (CFP)-10 of M.tb. antigens have been identified as vaccine candidates against Mycobacteria and used as subunit vaccines...
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BACKGROUND One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M. tuberculosis) genome sequence has led to identification of several antigens that can be utilized for accurate diagnosis and control of TB. The aim of this study was to purify the recombinant M. tuberculosis antigens for the eval...
متن کاملexpression and purification of recombinant mycobacterium tuberculosis (tb) antigens, esat-6, cfp-10 and esat- 6/cfp-10 and their diagnosis potential for detection of tb patients
متن کامل
quantiation of il-4, il-10 and ifn- genes expression after immunization of mice with cfp-10 and esat-6 containing vectors
background: tuberculosis is a disease with high morbidity, caused mainly by mycobaterium tuberculosis (m.tb.). dna vaccines show a promising future due to their unique advantages over conventional methods. the early-secreted antigen target (esat)-6 and culture filtrate protein (cfp)-10 of m.tb. antigens have been identified as vaccine candidates against mycobacteria and used as subunit vaccines...
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عنوان ژورنال:
reports of biochemistry and molecular biologyجلد ۲، شماره ۱، صفحات ۳۵-۴۱
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